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KMID : 0378019920350030045
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New Medical Journal
1992 Volume.35 No. 3 p.45 ~ p.54
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Chromosommal Changes in According to Culture Periods from β-lymphocytes Transdformed by Epstein-Barr virus
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Abstract
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Permanet lymphoblastoid cell lines are for great value in human clinical and experimental genetics. As not transformed cells have had limited life span, new materials were necessary in cases of reexamination. This might be difficult or even impossible, if patients were not at disposal for different reasons. Now a method for establishment of cell lines was developed by using Epstein-Barr virus (EBV) to B lymphocytes. This method has exhibited a number of advantages as compared to transformed fibroblast induced by Simian virus 40.
During the initial stage of EBV infection, cells expressed by EBNA appeared in increasing numbers and aggregated in foci of proliferative lymphoblast cells- However, cell death and complete degeneration of proliferative foci could be observed. This phenomenon proved to be dependent upon the presence of T cells in culture, namely, suppressor T or cytotoxic T or cytotoxic T cells affected to infected B cells by EBV.
Therefore T cells were removed by using nylon wool column method. This method is easier and little cytotoxic effect than T cells suppressing by cyclosporin A treatment
In cell line form two-months and one-year after culture with EBV, the modal number of SL (stem line) were 46 in 47% and in 59% of transformed cells respectively. With aging, the number of hypoteraploid cells were decreased. Conversely the number of hyperdiploidd cells were increased to 10°6. And chromosomal numbers which were gained in hyperdiploid cells were # 1, # 2. ?3, ? 9 and , ? 10. In structural anomalies of chromosomes the number of abnormal types were increased in one-year culture cells, that is these are t (8 : 14) (qter : qter), 15q-, t (10p: 13q). t (8q: 22q), b (3q), b (1), g (B) and dic (C).
Especially, the gap of chromosome 1 an 3 in EBV transformed cells was the same as nasopharyngeal cancer cells (Chang, et al, 1987). This indicates that EBV is an causative agent of nasopharyngeal cancer.
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